HIV-1 Transcription

Nuclear protein phosphatase-1 regulates HIV-1 transcription.

We recently reported that protein phosphatase 1 (PP1) dephosphorylates RNA polymerase II C-terminal repeats and regulates HIV-1 transcription in vitro. Here we provide evidence that PP1 is also required for Tat-induced HIV-1 transcription and for viral replication in cultured cells. Inhibition of PP1 by overexpression of nuclear inhibitor of PP1 (NIPP1) inhibited Tat-induced HIV-1 transcription...

Identification of HIV-1 Tat-Associated Proteins Contributing to HIV-1 Transcription and Latency

Human immunodeficiency virus type 1 (HIV-1) Tat is a virus-encoded trans-activator that plays a central role in viral transcription. We used our recently developed parallel analysis of in vitro translated open reading frames (ORFs) (PLATO) approach to identify host proteins that associate with HIV-1 Tat. From this proteomic assay, we identify 89 Tat-associated proteins (TAPs). We combine our re...

Effect of transcription peptide inhibitors on HIV-1 replication.

HIV-1 manipulates cellular machineries such as cyclin dependent kinases (cdks) and their cyclin elements, to stimulate virus production and maintain latent infection. Specifically, the HIV-1 viral protein Tat increases viral transcription by binding to the TAR promoter element. This binding event is mediated by the phosphorylation of Pol II by complexes such as cdk9/Cyclin T and cdk2/Cyclin E. ...

Topoisomerase II isoforms are required for HIV-1 reverse transcription

Background Human topoisomerase II is present in two forms 170kDa a and 180 kDa b isoform. Early reports have indicated that the topoII poisons and antisense RNA can block viral replication. The objective of present study is to evaluate specific requirement of topoII isomerases in promoting HIV replication and to characterize the HIV-1 replication intermediates to study molecular action of the t...

Apoptosis in HIV-1-Infected Macrophages Transcription Factor FOXO3a Mediates